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An efficient and eco-friendly workflow for dual fingermark processing and STR profiling
Yinon Harush-Brosh1, Lina Mayuoni-Kirshenbaum2, Yakov Mashiach2
1Institute of Dental Sciences, The Hebrew University - Hadassah School of Dental Medicine, POB 12272, Jerusalem, 9112102, Israel.
Forensic Science International. Genetics
|May 28, 2020
Summary
This study introduces a new, non-destructive method for collecting touch DNA and fingermarks (FM) simultaneously. The novel workflow enhances forensic identification by improving DNA recovery and fingermark visualization on various surfaces.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Background:
- Touch DNA and fingermark (FM) analysis are crucial in criminal casework.
- Current methods for FM processing can hinder subsequent DNA analysis.
- A novel, non-destructive workflow using gel-lifters and eco-friendly visualization was developed.
Purpose of the Study:
- To evaluate a new protocol for non-destructive fingermark collection and DNA recovery.
- To assess the protocol's effectiveness across different substrates, time delays, and donor genders.
- To determine the limitations and scope of the proposed workflow for forensic applications.
Main Methods:
- Developed 120 fingermarks from 20 donors (10 male, 10 female).
- Employed white BVDA gel-lifters and black Wetwop® solution for non-destructive FM collection and visualization.
- Recovered and analyzed DNA from both the gel-lifter adhesive and acetate imprint for each FM.
- Quantified DNA and performed genotyping, focusing on Short Tandem Repeat (STR) loci.
Main Results:
- The protocol demonstrated higher efficiency on non-porous substrates (e.g., 65% on car tin).
- Forensically useful DNA profiles were obtained from porous substrates like drywall (25% male, 15% female).
- Dual analysis of DNA from the gel-lifter and acetate imprint increased donor identification and confidence.
Conclusions:
- The novel workflow enables simultaneous, non-destructive collection of fingermarks and touch DNA.
- This integrated approach significantly enhances personal identification rates and strengthens forensic evidence.
- The protocol shows promise for improving casework efficiency in forensic investigations.
