Cross-linking/mass spectrometry at the crossroads.
Lolita Piersimoni1, Andrea Sinz2
1Department of Pharmaceutical Chemistry & Bioanalytics, Institute of Pharmacy, Charles Tanford Center, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3a, 06120, Halle (Saale), Germany.
Analytical and Bioanalytical Chemistry
|May 31, 2020
Summary
Cross-linking/mass spectrometry (XL-MS) now investigates protein interactions across entire proteomes, offering cellular process snapshots. This powerful technique provides structural insights into protein complexes, especially when combined with electron microscopy.
Area of Science:
- Biochemistry
- Proteomics
- Structural Biology
Background:
- Cross-linking/mass spectrometry (XL-MS) has evolved from studying small proteins to analyzing proteome-wide interactions.
- XL-MS provides dynamic snapshots of cellular processes.
- The technique is increasingly integrated into diverse biological research workflows.
Purpose of the Study:
- To provide an overview of the current state of XL-MS.
- To highlight the advantages of using MS-cleavable cross-linkers.
- To discuss future challenges and opportunities in XL-MS development.
Main Methods:
- XL-MS for mapping protein-protein interactions.
- Utilizing MS-cleavable cross-linkers.
- Integration with complementary structural biology techniques like electron microscopy.
Main Results:
- XL-MS enables the study of complex protein interactions on a proteome-wide scale.
- MS-cleavable cross-linkers facilitate data analysis and interpretation.
- Combined approaches yield valuable structural insights into protein complexes.
Conclusions:
- XL-MS is a rapidly advancing technology with growing impact in biological research.
- The technique offers significant benefits for understanding cellular mechanisms and protein complex structures.
- Future developments will further enhance the power and applicability of XL-MS.
Keywords:
Chemical cross-linkingCleavable cross-linkersElectron microscopyMass spectrometryProtein conformationProtein-protein interactionsStructural biologyMore Related Videos
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