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Updated: Dec 6, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

Light sheet fluorescence microscopy using axi-symmetric binary phase filters.

Suho Ryu^1,2, Baekcheon Seong^1,2, Chan-Wool Lee³

¹Department of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.

Biomedical Optics Express

|October 5, 2020

Summary

This summary is machine-generated.

This study introduces a new light sheet fluorescence microscopy (LSFM) method using binary phase filters (BPFs) to enhance the field-of-view (FoV) for clearer, deeper imaging in biomedical research.

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Last Updated: Dec 6, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

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Published on: October 28, 2018

Area of Science:

Biomedical Optics
Microscopy Techniques
Optical Engineering

Background:

Light sheet fluorescence microscopy (LSFM) offers depth-sectioning and low photobleaching for biological studies.
Axial resolution in LSFM depends on illumination sheet thickness, necessitating high numerical aperture lenses.
Illumination beam divergence limits the field-of-view (FoV), hindering high-resolution imaging over larger areas.

Purpose of the Study:

To develop an enhanced LSFM technique for improved field-of-view (FoV) and imaging performance.
To investigate the utility of binary phase filters (BPFs) derived from axi-symmetric aberrations for LSFM.
To overcome limitations of complex optical setups for generating specialized beams like Bessel or Airy beams.

Main Methods:

Integration of binary phase filters (BPFs) into the LSFM illumination path.
Utilizing BPFs derived from phase axicon and spherical aberrations.
Demonstrating imaging of microspheres, Drosophila larva imaginal discs, and Arabidopsis samples.

Main Results:

The novel LSFM approach significantly increased the effective field-of-view (FoV).
BPFs derived from axicon aberrations generated self-reconstructing beams, beneficial for imaging scattering specimens.
High-contrast, three-dimensional imaging was achieved over an extended FoV.

Conclusions:

Binary phase filters offer a practical and effective method for enhancing FoV in LSFM.
The developed LSFM technique improves imaging capabilities for complex biological samples.
This approach provides a valuable tool for advanced biomedical imaging research.