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THE CORRELATION OF THE CHEMICAL COMPOSITION OF ENAMEL AND ORAL FLUID IN PATIENTS WITH A WEDGE-SHAPED DEFECT AND
S Yarova1, I Zabolotna1, O Genzytska1
1Donetsk national medical university, Kramatorsk, Ukraine.
Abstract:
Aim - to determine oral fluid chemical composition and mineralization level in patients with a wedge-shaped defect and intact hard tissues, conduct correlation between the indicators of cervical enamel and oral fluid. We determined Сa2+, РО43-, SO42 using spectrophotometric method, Na+, К+, Al3+ by atomic emission spectrometry, Mg2+, Zn2+ by atomic absorption spectrometry, Cl- using chloride-selective electrode of pH-meter in oral fluid of 22 patients divided into two groups: case - with wedge-shaped defects (2.81+/- 0.73); control - with intact teeth. Correlation of oral fluid and cervical enamel chemical composition of 22 extracted teeth (12 - intact teeth, 10 wedge shape) was made. We used JSM-6490 LV focused beam electron microscope (scanning) with system of energy-dispersive X-ray microanalysis INCA Penta FETх3. Significant differences haven't been found in chemical analyte content and molar ratios (p>0.05) in oral fluid. The level of mineralization potential was 1.64+/-0.11 times higher in patients of the control group (p≤0.05). The values of molar coefficients in teeth enamel with a wedge-shaped defect were high: Mg/Р by 1.63 times, Ca/P by 24%, they were low: Mg/Са - by 4.7 times, Р/Са - by 14% (p≤0.05). The difference in correlation between the chemical composition of oral fluid and enamel of patients in the control group was the presence of an average directly proportional bond strength: Na+ with Cl- and Mg2+, inversely proportional: Na+ with Сa2+ and РО43-, р<0.0001. Correlation between molar coefficients of oral fluid and enamel in the control group was distinguished by the presence of high and moderate correlation: directly proportional - between Са2+/Cl- and Na+/К+, Са2+/Mg2+; Mg2+/Са2+ and РО43-/Са2+, inversely proportional - between Са2+/Mg2+ and РО43-/Са2+, р<0.0001. We have revealed correlations of the chemical composition of enamel and oral fluid which can be used to assess the mineral state of tooth enamel and control the effectiveness of prevention of the initial forms of wedge-shaped defects in terms of mixed saliva in dynamics.
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