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In-Line Microelectrode Arrays for Impedance Mapping of Microphysiological Systems
Ashlyn T Young1, Vladimir A Pozdin2, Michael Daniele1,3
1Joint Department of Biomedical Engineering, NC State University and UNC at Chapel Hill, Raleigh, NC, USA.
Proceedings of IEEE Sensors. IEEE International Conference on Sensors
|March 31, 2021
Summary
A novel 60-electrode array was fabricated for microphysiological systems, enabling detailed impedance mapping of microchannels. This technology provides high spatial resolution for analyzing cellular environments and fluidic systems.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Electrochemical Analysis
Background:
- Microphysiological systems (MPS) require advanced analytical tools for real-time monitoring.
- Characterizing microenvironments within MPS is crucial for understanding biological processes.
- Existing methods may lack the spatial resolution needed for detailed analysis.
Purpose of the Study:
- To develop and demonstrate a high-resolution electrode array for impedance analysis in microchannels.
- To create an impedance mapping technique for microphysiological systems.
- To validate the system's capability using controlled solutions and cell cultures.
Main Methods:
- Fabrication of a 60-electrode array using photolithography, metallization, and etching.
- Impedance measurements (10 Hz to 1 MHz) along a microchannel using a multiplexer and potentiostat.
- Development of custom algorithms to generate impedance "heat maps".
Main Results:
- Successful fabrication of a 60-electrode array integrated into a microchamber.
- Demonstration of 2-wire impedance measurements across the electrode array.
- Generation of spatial impedance maps using conductive NaCl solutions.
- Validation of mapping capabilities with 2D cell cultures, showing distinct impedance profiles.
Conclusions:
- The developed 60-electrode array provides a powerful tool for spatially resolved impedance analysis in microfluidic devices.
- Impedance mapping offers a non-invasive method to characterize microenvironments within microphysiological systems.
- This technology has potential applications in drug screening, disease modeling, and fundamental biological research.
