Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-Wide Single-Nucleotide Resolution Mapping of DNA Damage by Dual Enzyme Cleavage-Assisted Sequencing.

Analytical chemistry·2026
Same author

DEHP disrupts lipid metabolism via autophagy hyperactivation and mitochondrial dysfunction.

Autophagy·2026
Same author

Polymerase κ Recruits DDX23 To Promote R-Loop Resolution.

Analytical chemistry·2026
Same author

Landscape of environmental pollutant-driven alterations of nucleic acid modifications in hepatoma cells.

Journal of hazardous materials·2026
Same author

A super protein evolution engine.

Nature chemical biology·2025
Same author

Call for Papers: Special Issue on Nucleic Acid Modifications.

Chemical research in toxicology·2025
Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026
Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026
Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026
Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026
Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Nov 10, 2025

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

8.9K

Quantitative Analysis of Oncometabolite 2-Hydroxyglutarate.

Bi-Feng Yuan1

  • 1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China. bfyuan@whu.edu.cn.

Advances in Experimental Medicine and Biology
|April 1, 2021
PubMed
Summary
This summary is machine-generated.

Gain-of-function mutations in isocitrate dehydrogenase 1 and 2 (IDH1/2) produce oncometabolite 2-hydroxyglutarate (2HG), increasing cancer risk. This review details methods for analyzing 2HG enantiomers (D-2HG and L-2HG) crucial for disease diagnosis.

Keywords:
2-HydroxyglutarateChiral derivatizationEnantiomerGas chromatography-mass spectrometryLiquid chromatography-mass spectrometryOncometabolite

More Related Videos

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.4K
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.4K

Related Experiment Videos

Last Updated: Nov 10, 2025

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

8.9K
One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.4K
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.4K

Area of Science:

  • Biochemistry
  • Metabolomics
  • Oncology

Background:

  • Gain-of-function mutations in isocitrate dehydrogenase 1 and 2 (IDH1/2) lead to the accumulation of the oncometabolite 2-hydroxyglutarate (2HG).
  • 2HG competes with α-ketoglutarate (α-KG), inhibiting critical α-KG-dependent dioxygenases involved in cellular metabolism and epigenetics.
  • Elevated 2HG levels are associated with an increased risk of malignant tumors.

Purpose of the Study:

  • To review and discuss established quantitative methods for analyzing total 2-hydroxyglutarate (2HG).
  • To focus on the determination of the specific enantiomers, D-2-hydroxyglutarate (D-2HG) and L-2-hydroxyglutarate (L-2HG).
  • To highlight the importance of enantiomeric analysis for accurate diagnosis of 2HG-related diseases.

Main Methods:

  • Utilizing chiral chromatography to separate D-2HG and L-2HG enantiomers before spectroscopic or mass spectrometry analysis.
  • Employing chiral derivatization reagents to convert enantiomers into diastereomers for improved chromatographic separation.
  • Summarizing established analytical strategies for both total 2HG and its individual enantiomers.

Main Results:

  • Quantitative methods for analyzing total 2HG are well-developed.
  • Established strategies exist for the accurate determination of D-2HG and L-2HG enantiomers.
  • These methods enable precise measurement critical for clinical diagnosis.

Conclusions:

  • Accurate diagnosis of 2HG-related diseases necessitates determining the specific enantiomeric configuration (D-2HG vs. L-2HG).
  • A range of quantitative analytical methods, primarily based on chiral separation techniques, are available.
  • This review consolidates current knowledge on 2HG enantiomer analysis, supporting diagnostic advancements.