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Published on: March 4, 2018
Exogenous ATP in the Cryopreservation of Brycon orbignyanus Spermatozoa
C T Perry1, C D Corcini2, I B Acosta2
1Federal University of Rio Grande, Biology of Continental Aquatic Environments. Rio Grande, Brazil.
Background:
ATP exogenous (ATPe) has been used successfully in improving motility and fertility for many animal species. However this has not yet been tested on Brycon orbignyamus.
Objective:
The objective of this study was to evaluate the use of ATPe for the cryopreservation of sperm from B. orbignyamus.
Materials And Methods:
The ATPe concentrations tested were 1.0 µM, 5.0 µM and 10 µM combined with Beltsville Thawing SolutionTM extender and dimethylformamide at 7.5%. The sperm were frozen in a nitrogen vapour vessel and stored in liquid nitrogen at -196 ºC. The parameters of viability post-thawing were evaluated using CASA, and flow cytometer.
Results:
The ATPe did not promote improvements in spermatic kinetics, and in the higher concentrations caused a worsening in these parameters. Also there was loss of mitochondrial functionality and greater cellular disruption with the concentration of 10 μM.
Conclusion:
We do not recommend the addition of ATP for cryopreserving B. orbignyamus.
