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Updated: Oct 19, 2025

Dissection and Culture of Mouse Embryonic Kidney
Published on: May 17, 2017
Connection of ES Cell-derived Collecting Ducts and Ureter-like Structures to Host Kidneys in Culture
May Sallam1,2, Jamie Davies1
1Deanery of Biomedical Science, University of Edinburgh, Edinburgh, UK.
Abstract:
Work toward renal generation generally aims either to introduce suspensions of stem cells into kidneys in the hope that they will rebuild damaged tissue, or to construct complete new kidneys from stem cells with the aim of transplanting the engineered organs. In principle, there might be a third approach; to engineer renal tissue 'modules' in vitro and to use them to replace sections of damaged host kidney. This approach would require the urine collecting system or ureter of the new tissues to connect to those of the host. In this report, we demonstrate a method that allows collecting duct trees or ureters, engineered from ES cells, to connect to the collecting duct system or ureter, respectively, of fetal kidneys in culture.
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