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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

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Published on: July 5, 2016

Handheld and Cost-Effective Fourier Lightfield Microscope.

Laura Galdon¹, Hui Yun¹, Genaro Saavedra¹

¹3D Imaging and Display Laboratory, Department of Optics, Universidad de Valencia, 46100 Burjassot, Spain.

Sensors (Basel, Switzerland)

|February 26, 2022

Summary

This summary is machine-generated.

Researchers developed a portable Fourier Lightfield Microscope (FLMic) using affordable parts. This handheld device offers 3D imaging and refocusing capabilities at a fraction of the cost of traditional microscopes.

Keywords:

3D imaging light-field microscopy

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Area of Science:

Optical microscopy
Biomedical imaging
Instrumentation

Background:

Lightfield microscopy enables 3D imaging and refocusing with a single shot.
Existing Fourier Lightfield Microscopes (FLMic) are often expensive and not portable.
There is a need for cost-effective and field-deployable 3D imaging solutions.

Purpose of the Study:

To design, build, and test a highly portable, cost-effective Fourier Lightfield Microscope (FLMic).
To demonstrate the feasibility of using off-the-shelf components for advanced microscopy.
To provide a robust 3D imaging solution for in-field applications.

Main Methods:

Construction of the FLMic using a surveillance camera lens and common optical elements.
Integration of components to achieve a compact and lightweight handheld system.
Testing the system's operability in bright-field and fluorescent modes.

Main Results:

The developed FLMic is significantly more portable, easier to build, and cost-effective than conventional systems.
The system successfully performs 3D imaging and refocusing using a single-shot approach.
Optical performance is minimally impacted despite the use of low-cost components, achieving over 100x cost reduction.

Conclusions:

The proposed FLMic is a strong candidate for in-field 3D imaging due to its portability, low cost, and robust performance.
This design democratizes access to advanced lightfield microscopy capabilities.
Further applications in diverse fields requiring accessible 3D imaging are anticipated.