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Related Concept Videos

Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Cell Diversity01:13

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The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
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Related Experiment Video

Updated: Oct 1, 2025

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

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Human 8-cell-like cells discovered.

Bradley P Balaton1, Vincent Pasque1

  • 1KU Leuven-University of Leuven, Department of Development and Regeneration, Leuven Institute for Single-Cell Omics (LISCO), Leuven Stem Cell Institute, Leuven, Belgium.

Cell Stem Cell
|March 4, 2022
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Summary
This summary is machine-generated.

Human naive pluripotent stem cells can form blastoids. Researchers discovered these stem cell cultures contain cells mirroring the 8-cell human embryo, enabling studies on early gene activation.

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Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts
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Area of Science:

  • Developmental biology
  • Stem cell research
  • Human embryogenesis

Background:

  • Human naive pluripotent stem cells (hPSCs) possess self-renewal and differentiation capabilities.
  • hPSCs can model early human development, including blastoid formation.
  • Understanding early human embryonic gene activation is crucial for developmental studies.

Purpose of the Study:

  • To investigate the presence of early human embryo-like cells within human naive pluripotent stem cell cultures.
  • To establish a novel model for studying zygotic genome activation in humans.

Main Methods:

  • Culture of human naive pluripotent stem cells.
  • Characterization of cell populations using microscopy and molecular markers.
  • Comparison of cell morphology and gene expression with early human embryos.

Main Results:

  • Human naive pluripotent stem cell cultures contain cells resembling the 8-cell human embryo.
  • These cells exhibit characteristics necessary for studying zygotic genome activation.
  • The findings provide a new avenue for modeling human pre-implantation development.

Conclusions:

  • Human naive pluripotent stem cells offer a unique resource for studying early human development.
  • The identified 8-cell embryo-like cells in hPSC cultures are a valuable model for zygotic genome activation research.
  • This discovery advances the study of human embryogenesis and related disorders.