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A CDK activity buffer ensures mitotic completion.

Souradeep Basu1, James O Patterson1, Theresa U Zeisner1

  • 1Cell Cycle Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

Journal of Cell Science
|June 21, 2022
PubMed
Summary
This summary is machine-generated.

Cells can tolerate reduced cyclin-dependent kinase (CDK) activity during mitosis. A CDK activity buffer ensures mitotic completion, preventing lethality until this buffer is depleted.

Keywords:
Schizosaccharomyces pombeCDK inhibitorCdk1Cyclin-dependent kinaseKinase activityMitosis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The eukaryotic cell cycle is regulated by cyclin-dependent kinases (CDKs).
  • CDK activity increases significantly from G1 to mitosis.
  • The necessity of the full range of CDK activity for cell cycle progression remains unclear.

Purpose of the Study:

  • To investigate whether cells can tolerate reduced CDK activity levels.
  • To determine the role of CDK activity levels in mitotic progression and completion.
  • To identify factors influencing the CDK activity buffer.

Main Methods:

  • Experiments were conducted using fission yeast.
  • Sublethal CDK inhibition was applied to observe effects on mitosis.
  • Factors influencing the CDK activity buffer, including phosphatases, were analyzed.

Main Results:

  • Sublethal CDK inhibition prolongs mitosis duration without disrupting event order.
  • Maximum CDK activity exceeds the level required for mitosis, creating an activity buffer.
  • This buffer is crucial for mitotic completion under compromised CDK activity.
  • CDK inhibition becomes lethal only when the activity buffer is exhausted.

Conclusions:

  • Maximum attainable CDK activity is not essential for mitosis itself.
  • A CDK activity buffer provides robustness against CDK activity reduction, ensuring mitotic completion.
  • CDK-counteracting phosphatases influence the size of this activity buffer.