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Related Experiment Video

Updated: Sep 2, 2025

A Microfluidic Platform to Study Bioclogging in Porous Media

A Microfluidic Platform to Study Bioclogging in Porous Media

Published on: October 13, 2022

A 3D-Printed Customizable Platform for Multiplex Dynamic Biofilm Studies.

Atul Dhall¹, Ravikiran Ramjee², Min Jun Oh³

¹Department of Preventive and Restorative Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Advanced Materials Technologies

|August 8, 2022

Summary

This summary is machine-generated.

This study introduces a 3D-printed platform for advanced biofilm research. The customizable system enables real-time monitoring of microbial communities under dynamic conditions, improving experimental strategies.

Keywords:

3D printing biofilms hydrodynamics multiplex system real-time pH measurement

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Last Updated: Sep 2, 2025

A Microfluidic Platform to Study Bioclogging in Porous Media

A Microfluidic Platform to Study Bioclogging in Porous Media

Published on: October 13, 2022

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Microfabricated Platforms for Mechanically Dynamic Cell Culture

Published on: December 26, 2010

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Published on: May 16, 2019

Area of Science:

Microbiology
Biotechnology
Bioengineering

Background:

Biofilms are microbial communities that colonize surfaces, crucial in various environments.
Existing biofilm models often lack accurate spatiotemporal dynamics replication.
Understanding complex biofilm development requires advanced experimental tools.

Purpose of the Study:

To present a novel, customizable 3D-printed platform for versatile biofilm monitoring.
To enable real-time tracking of microbial adhesion, growth, and associated parameters.
To overcome limitations of current biofilm experimental models.

Main Methods:

A 3D-printed platform compatible with standard well plates was developed.
The system supports substrates in vertical positions, variable flow angles, and mode switching (static/dynamic).
Integrated peristaltic pump, pH monitoring, and fluid dynamics simulations were utilized.

Main Results:

The platform successfully monitored biofilm development under static and dynamic conditions using *Streptococcus mutans*.
Effluent pH and biofilm properties (biochemical, microbiological, morphological) were tracked in real-time.
Distinct biofilm properties were demonstrated under varied experimental conditions.

Conclusions:

The novel 3D-printed platform offers a versatile solution for biofilm research.
It enhances the ability to design and execute complex biofilm investigations.
This technology advances the study of microbial communities in diverse scenarios.