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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Aug 30, 2025

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
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Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

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Flow Cytometry and Cell Cycle Analysis: An Overview.

Aja M Rieger1

  • 1Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada. aja@ualberta.ca.

Methods in Molecular Biology (Clifton, N.J.)
|August 31, 2022
PubMed
Summary
This summary is machine-generated.

Flow cytometry cell cycle analysis, a long-standing technique, has evolved significantly. Advances in DNA dyes and technology continue to drive innovation in this dynamic field.

Keywords:
Cell cycleDNA contentDNA content dyesFlow cytometry

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Cell cycle analysis is a foundational application of flow cytometry, established with early Feulgen-DNA staining methods.
  • The field has continuously evolved, integrating new technologies and a deeper understanding of cell cycle phase markers.

Purpose of the Study:

  • To provide a comprehensive overview of the current advancements and methodologies in cell cycle analysis using flow cytometry.
  • To highlight the dynamic nature of this research area within the broader flow cytometry community.

Main Methods:

  • Utilizes flow cytometry, a technology for analyzing physical and chemical characteristics of cells.
  • Incorporates advanced DNA staining techniques and the identification of novel cell cycle phase markers.

Main Results:

  • Demonstrates the sustained relevance and ongoing development of flow cytometry for cell cycle studies.
  • Highlights the impact of recent technological and methodological improvements on the field.

Conclusions:

  • Flow cytometry remains a critical tool for cell cycle analysis, benefiting from continuous innovation.
  • The field is characterized by its dynamic evolution, driven by new dyes, markers, and technologies.