Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Accurate Lens-Distortion Measurement Through Detector Nyquist Sampling.

Sensors (Basel, Switzerland)·2026
Same author

Differential Confocal Optical Probes with Optimized Detection Efficiency and Pearson Correlation Coefficient Strategy Based on the Peak-Clustering Algorithm.

Micromachines·2023
Same author

A Differential Confocal Sensor for Simultaneous Position and Slope Acquisitions Based on a Zero-Crossing Prediction Algorithm.

Sensors (Basel, Switzerland)·2023
Same author

Factors Influencing Vaccine Hesitancy in China: A Qualitative Study.

Vaccines·2021
Same author

Deep-Stacking Network Approach by Multisource Data Mining for Hazardous Risk Identification in IoT-Based Intelligent Food Management Systems.

Computational intelligence and neuroscience·2021
Same author

Laparoscopic onlay lingual mucosal graft ureteroplasty combined with ureterovesical reimplantation for one-stage reconstruction of complex ureteral strictures: a case report.

Translational andrology and urology·2021

Related Experiment Video

Updated: Aug 27, 2025

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

2.5K

Precise Two-Dimensional Tilt Measurement Sensor with Double-Cylindrical Mirror Structure and Modified Mean-Shift

Zhiyi Wang1,2, Tingyu Wang1,2, Yongqiang Yang1,2

  • 1Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.

Sensors (Basel, Switzerland)
|September 23, 2022
PubMed
Summary

A novel double-cylindrical mirror sensor enhances confocal microscopy for precise 3D optical surface measurements. This system improves accuracy and speed in freeform optics contour detection.

Keywords:
confocal microscopyoptical profilometrypartition-fitting strategypeak-extraction algorithmpositiontwo-dimensional tilt

More Related Videos

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.0K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.0K

Related Experiment Videos

Last Updated: Aug 27, 2025

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces
08:05

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Published on: September 9, 2022

2.5K
Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

28.0K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.0K

Area of Science:

  • Optical Metrology
  • Microscopy Systems
  • Surface Characterization

Background:

  • Accurate three-dimensional (3D) surface contour measurement of freeform optics is critical for advanced applications.
  • Traditional confocal microscopy (CM) systems face challenges in achieving high precision due to factors like object reflectivity and light source instability.
  • Existing methods often struggle with simultaneous measurement of inclination and spatial coordinates.

Purpose of the Study:

  • To develop and validate a novel two-dimensional (2D) tilt measurement sensor for CM systems.
  • To enhance the accuracy, robustness, and speed of 3D surface contour measurements for freeform optics.
  • To enable simultaneous estimation of 2D inclination angle and spatial position coordinates.

Main Methods:

  • A double-cylindrical mirror structure was designed and modeled for the 2D tilt measurement sensor.
  • A modified mean-shift-based peak-extraction algorithm with a novel kernel function (MSN) was introduced to address measurement inaccuracies.
  • A partition fitting (PF) strategy was implemented to minimize fitting errors and improve overall measurement precision.

Main Results:

  • Simulations and experiments demonstrated significant improvements in the sensor's robustness, speed, and angular prediction accuracy.
  • The developed sensor achieved mean prediction errors of 0.0134° (0.067% F.S) and 0.0142° (0.071% F.S) in the 2D plane over a -10° to +10° range.
  • The system successfully enabled simultaneous estimation of 2D inclination angle and spatial position coordinates.

Conclusions:

  • The proposed double-cylindrical mirror sensor integrated with MSN and PF strategies offers a substantial advancement in CM-based 3D surface metrology.
  • The sensor provides accurate and simultaneous 2D tilt and positional data, crucial for precise surface profiling.
  • This technology holds significant potential for applications in 3D topographic reconstruction and optimizing sampling rates in 3D contour detection.