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Engineering Sandwiched Nanochannel Aptasensor for Efficiently Screening Cancer Cells
Rui Lv1, Xing Wang1, Zhiqiang Mao1
1Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and, Application of Organic Functional Molecules, College of Health Sciences and Engineering, Hubei University, Wuhan, 430062, P. R. China.
A new aptamer sandwiched nanochannel electrochemical sensor offers sensitive and selective cancer cell detection. This method achieves a low limit of detection for breast cancer cells (MCF-7), aiding in early diagnosis.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Electrochemistry
Background:
- Cancer cells are crucial tumor biomarkers linked to disease progression.
- Accurate and rapid cancer cell detection methods are urgently needed.
- Existing methods may face challenges like electrode passivation.
Purpose of the Study:
- To develop a sensitive and highly efficient aptamer sandwiched nanochannel electrochemical sensor.
- To achieve highly selective determination of cancer cells.
- To establish a reliable platform for cancer cell screening.
Main Methods:
- Fabrication of a nanochannel-based electrochemical sensor.
- Immobilization of DNA aptamers within porous nanochannels.
- Utilizing a sandwich structure for specific cancer cell capture and detection.
- Electrochemical analysis for MCF-7 cell screening.
Main Results:
- The developed sensor demonstrated excellent performance for cancer cell determination.
- Achieved a low limit of detection of 36 cells/mL for MCF-7 cells.
- The nanochannel structure effectively filtered and captured target cells.
- The sensor design prevented electrode passivation during analysis.
Conclusions:
- The aptamer sandwiched nanochannel electrochemical sensor provides an effective and reliable approach for cancer cell detection.
- This method offers high sensitivity and selectivity for tumor biomarker analysis.
- The technology shows promise for advancing early cancer diagnosis and monitoring.
