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Updated: Aug 15, 2025
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In Situ Ca2+ Imaging of the Enteric Nervous System
Published on: January 29, 2015
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Identifying Types of Neurons in the Human Colonic Enteric Nervous System.
Simon Brookes1, Nan Chen2, Adam Humenick2
1Human Physiology, College of Medicine and Public Health, Flinders University, Adelaide, SA, USA. simon.brookes@flinders.edu.au.
Advances in Experimental Medicine and Biology
|December 31, 2022
Summary
Researchers developed a novel method to classify human enteric neurons using multiple markers. This technique enhances our understanding of the human colon
Area of Science:
- Neuroscience
- Gastroenterology
- Cell Biology
Background:
- Classifying enteric nervous system (ENS) neurons is crucial for understanding gut function.
- Human ENS studies lag behind animal models despite tissue availability.
- Single-cell sequencing advances ENS neuron classification but an encompassing system is lacking.
Purpose of the Study:
- To present preliminary data on a method distinguishing human myenteric neuron classes.
- To combine immunohistochemical, morphological, projection, and size data for single-cell analysis.
- To provide a comprehensive account of human colon myenteric neuron types.
Main Methods:
- Developed a multi-layer antiserum application method for up to 12 markers per neuron.
- Applied the method to specimens of human colon myenteric neurons.
- Combined immunohistochemistry, morphology, projection, and size data.
Main Results:
- Dogiel type II neurons constitute <5% of myenteric neurons.
- These neurons are immunoreactive for choline acetyltransferase and tachykinins.
- Many express calbindin and calretinin and are larger than average.
Conclusions:
- The developed methodology complements single-cell mRNA profiling.
- It offers a comprehensive approach to classifying human colon myenteric neurons.
- This advances the characterization of the human enteric nervous system.
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