Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell survival and plating efficiency.

D J Brenner1, M Zaider, C R Geard

  • 1Radiological Research Laboratory, College of Physicians and Surgeons of Columbia University, New York, New York 10032.

Radiation Research
|September 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Traceable dosimetry for MeV ion beams.

Journal of instrumentation : an IOP and SISSA journal·2022
Same author

Proton Irradiation Platforms for Preclinical Studies of High-Dose-Rate (FLASH) Effects at RARAF.

Radiation research·2020
Same author

LET dependent response of GafChromic films investigated with MeV ion beams.

Physics in medicine and biology·2018
Same author

A Horizontal Multi-Purpose Microbeam System.

Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment·2018
Same author

Mathematical modelling of scanner-specific bowtie filters for Monte Carlo CT dosimetry.

Physics in medicine and biology·2017
Same author

THE DECADE OF THE RABiT (2005-15).

Radiation protection dosimetry·2016
Same journal

KRT6A Impairs Radiosensitivity in Cervical Squamous Cell Carcinoma by Enhancing Fatty Acid Synthesis.

Radiation research·2026
Same journal

Chromosomal Instability: A Potential Biomarker of Radiation Response.

Radiation research·2026
Same journal

Antioxidant Probucol Reduces Mortality in Mice Exposed to Lethal Doses of Ionizing Radiation.

Radiation research·2026
Same journal

The Detection of Radiation Effects in the Urine of Rhesus Macaques Using Raman Spectroscopy.

Radiation research·2026
Same journal

Characterization of Radiation-responsive Genes and Transcript Variants under Different Radiation Qualities, Doses and Dose Rates.

Radiation research·2026
Same journal

Methyl Quercetin Inhibits Radiation-induced Senescence and TGF-β1-induced Myofibroblast Differentiation Through Psmad3/TGF-Β Signaling.

Radiation research·2026
See all related articles

Cellular radiation sensitivity correlates with plating efficiency (PE) in human and rodent cell lines. This relationship holds true even when accounting for passage number in mortal cells, suggesting PE is a key factor in radiation response.

Area of Science:

  • Cell Biology
  • Radiation Oncology
  • Genetics

Background:

  • Cellular radiation sensitivity is a critical factor in radiotherapy and radiobiology.
  • Plating efficiency (PE), a measure of cell survival and growth, is often used in cell culture experiments.
  • The relationship between cellular radiation sensitivity and PE requires further clarification.

Purpose of the Study:

  • To investigate the correlation between cellular radiation sensitivity and plating efficiency (PE).
  • To determine if this correlation is consistent across different cell types (human vs. rodent, mortal vs. immortal).

Main Methods:

  • Utilized three distinct cell lines: human mortal (AG 1522), rodent immortal (CHO AA8), and rodent mortal (C3H 10T1/2).
  • Assessed cellular radiation sensitivity and plating efficiency for each cell line.

Related Experiment Videos

  • Accounted for the effect of passage number in mortal cell lines.

    • Main Results:

    • A strong positive correlation between radiation sensitivity and PE was observed in the human mortal (AG 1522) and rodent immortal (CHO AA8) cell lines.
    • This correlation persisted even after controlling for passage number in the mortal cell line (AG 1522).

    Conclusions:

    • Cellular radiation sensitivity is closely related to plating efficiency (PE).
    • PE appears to be a significant determinant of radiation response across different cell types.
    • Findings suggest PE should be considered when evaluating cellular radiosensitivity.