Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.5K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of Complex Liquid-Liquid Extraction Organic Phases for Rare Earth Separations.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

<i>LaueMatching</i>: an approach for rapid and robust indexing of Laue diffraction patterns.

Journal of applied crystallography·2026
Same author

Optimizing coded-apertures for depth-resolved diffraction.

The Review of scientific instruments·2025
Same author

Nano-laminography with a transmission X-ray microscope.

Journal of synchrotron radiation·2025
Same author

Measurements of source emittance and beam coherence properties of the upgraded Advanced Photon Source.

Journal of synchrotron radiation·2025
Same author

Extracted Water Induces Concentration Fluctuations in Model Ternary Liquid-Liquid Extraction System.

The journal of physical chemistry. B·2025
Same journal

Compressed multi-scale entropy and its application in mechanical fault diagnosis.

The Review of scientific instruments·2026
Same journal

Bidirectional drive and multi-resolution adjustment across frequency bands in inertial impact piezoelectric motors via multimodal resonant vibration.

The Review of scientific instruments·2026
Same journal

A magnetic field sensor based on flaky Terfenol-D material and dual fiber grating.

The Review of scientific instruments·2026
Same journal

A novel E-field eight-way cavity combiner for high-power S-band applications.

The Review of scientific instruments·2026
Same journal

Constant radius blade spring suspended bench for vibration isolation.

The Review of scientific instruments·2026
Same journal

Qualification of infrared optical fibers and emitters for a spectrometer for in situ planetary exploration: Results from the TRIS (TRansmission and Illumination System) project.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Aug 12, 2025

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.4K

Digital autofocusing of a coded-aperture Laue diffraction microscope.

Doğa Gürsoy1, Dina Sheyfer1, Michael Wojcik1

  • 1X-ray Science Division, Argonne National Laboratory, 9700 S Cass Ave., Lemont, Illinois 60439, USA.

The Review of Scientific Instruments
|February 1, 2023
PubMed
Summary
This summary is machine-generated.

Accurately positioning a coded-aperture microscope is crucial for high-resolution imaging. This study introduces an automated optimization method to precisely determine the coded-aperture geometry, improving efficiency in Laue diffraction microscopy.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.1K

Related Experiment Videos

Last Updated: Aug 12, 2025

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.4K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

12.1K

Area of Science:

  • Materials Science
  • Microscopy
  • Crystallography

Background:

  • Optimal depth resolution in coded-aperture Laue diffraction microscopy requires precise knowledge of the coded-aperture position and scanning geometry.
  • Determining this geometry through trial and error is inefficient due to the numerous parameters involved.

Purpose of the Study:

  • To develop and validate an automated optimization approach for determining coded-aperture geometry.
  • To enhance the efficiency and accuracy of focusing in coded-aperture Laue diffraction microscopy.

Main Methods:

  • An optimization algorithm was developed to automate the determination of coded-aperture position and scanning geometry.
  • The method processes collected data to refine the geometric parameters.

Main Results:

  • The proposed optimization approach successfully automates the focusing process.
  • Demonstrated robustness and efficiency using experimental data from a synchrotron facility.

Conclusions:

  • Automated optimization significantly reduces the time and effort required to determine microscope geometry.
  • This approach offers a more efficient and reliable method for achieving optimal depth resolution in coded-aperture Laue diffraction microscopy.