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Some steric aspects of synthesis of oligoribonucleotides by phosphoroamidite approach on solid support
R Kierzek1, M Rozek, W T Markiewicz
1Institute of Bio-organic Chemistry, Polish Academy of Sciences, Poznan.
Nucleic Acids Symposium Series
|January 1, 1987
Abstract:
The influence of 2'-O-substituents (i.e. methyl, tetrahydropyranyl, t-butyldimethylsilyl) on the chemical synthesis of oligoribonucleotides by the phosphoroamidite approach on solid support is studied and compared with 2'-deoxynucleosides. Strong dependence on the steric hindrance of 2'-substituents both for the 3'-phosphitylation and condensation steps is described.