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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Cell Lines01:16

Cell Lines

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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Related Experiment Video

Updated: Jul 29, 2025

Working with Auditory HEI-OC1 Cells
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Working with Auditory HEI-OC1 Cells

Published on: September 3, 2016

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HEV Cell Culture.

Feng Zhang1, Youchun Wang2

  • 1Division of Therapeutical Monoclonal Antibodies, National Institutes for Food and Drug Control, Beijing, China.

Advances in Experimental Medicine and Biology
|May 24, 2023
PubMed
Summary
This summary is machine-generated.

Hepatitis E virus (HEV) research benefits from efficient cell culture systems and infectious cDNA clones. These methods help study HEV virulence, host range, and protein functions, advancing HEV diagnostics and therapeutics.

Keywords:
Cell cultureCytopathic effectPassageTissue culture infective dose

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Area of Science:

  • Virology
  • Hepatitis E Virus (HEV) Research

Background:

  • Cell culture is crucial for virology research, but efficient Hepatitis E virus (HEV) culturing remains challenging.
  • Factors like virus stock concentration, host cells, and medium components influence HEV culture efficiency.
  • Genetic mutations during HEV passage can increase virulence in cell culture.

Purpose of the Study:

  • To explore efficient cell culture systems for HEV.
  • To investigate HEV properties using infectious cDNA clones as an alternative to traditional cell culture.
  • To understand HEV protein functions and virus assembly.

Main Methods:

  • Development and utilization of efficient HEV cell culture systems.
  • Construction and application of infectious HEV cDNA clones.
  • Analysis of HEV progeny virus characteristics in cell culture.

Main Results:

  • Infectious cDNA clones enabled studies on HEV thermal stability, host range, and protein functions.
  • HEV cell culture revealed that secreted viruses possess an envelope.
  • The pORF3 protein was identified as crucial for HEV envelope formation.

Conclusions:

  • Efficient HEV cell culture and infectious cDNA clones are valuable research tools.
  • Understanding HEV assembly, particularly the role of pORF3, offers insights into HEV infection mechanisms.
  • This research contributes to developing better HEV diagnostics and treatments.