Related Experiment Video
Updated: Jul 27, 2025
High Throughput Microinjections of Sea Urchin Zygotes
Published on: January 21, 2014
The Time-Course of Hatching Enzyme Secretion in the Sea Urchin Strongylocentrotus purpuratus:
Thomas D Yager1, Dennis Barrett2
1Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA.
Abstract:
By two independent methods, we have determined approximately the time-course of hatching enzyme secretion in the sea urchin Strongylocentrotus purpuratus. A quick-kill method indicates that a significant fraction of the enzyme is secreted between 90% and 97% of the fertilization-hatching interval. A direct assay method indicates that the remainder of the enzyme is secreted on either side of the 90-97%"window". The entire period of secretion spans from 75% to 100% or more of the fertilization-hatching interval. For embryos raised at 15°C this translates to an interval of 4.8 or more hr.
