Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Label-Free Dual-Mode Flow Cytometry for Single-Cell Physicochemical Property Analysis.

Analytical chemistry·2026

Same author

DLD-based on-chip sample handling for label-free single-cell MS cytometry.

Analytical methods : advancing methods and applications·2025

Same author

Neuromorphic-enabled video-activated cell sorting.

Nature communications·2024

Same author

Liquid metal electrodes enabled cascaded on-chip dielectrophoretic separation of large-size-range particles.

Lab on a chip·2024

Same author

Microfluidic Impedance Cytometry Enabled One-Step Sample Preparation for Efficient Single-Cell Mass Spectrometry.

Small (Weinheim an der Bergstrasse, Germany)·2024

Same author

Capillarity Enabled Large-Array Liquid Metal Electrodes for Compact and High-Throughput Dielectrophoretic Microfluidics.

Advanced materials (Deerfield Beach, Fla.)·2024

Search research articles

Related Experiment Video

Updated: Jul 23, 2025

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

Impedance-Based Multimodal Electrical-Mechanical Intrinsic Flow Cytometry.

Yongxiang Feng¹, Junwen Zhu¹, Huichao Chai¹

¹State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, 100190, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)

|July 12, 2023

Summary

This study introduces a novel electrical-mechanical flow cytometry method for precise single-cell analysis. This label-free technique accurately distinguishes cell types and drug effects using intrinsic biophysical properties.

Keywords:

electrical-mechanical properties impedance flow cytometry intrinsic biophysical properties intrinsic multimodal characterization single cell analysis

More Related Videos

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Related Experiment Videos

Last Updated: Jul 23, 2025

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Area of Science:

Biophysics
Cell Biology
Biotechnology

Background:

Intrinsic biophysical properties (mechanical, electrical) are key cell biomarkers.
Single-modal characterization lacks specificity; multimodal analysis is challenging.

Purpose of the Study:

To develop an impedance-based multimodal electrical-mechanical flow cytometry framework.
To enable on-the-fly, high-dimensional intrinsic measurement of single cells.

Main Methods:

Leveraged spatial-temporal impedance sensing signal characteristics.
Integrated electrical and mechanical measurements for comprehensive cell profiling.
Measured Young's modulus (E), fluidity (β), radius (r), cytoplasm conductivity (σi), and membrane capacitance (Csm).

Main Results:

Achieved 93.4% accuracy in classifying three cancer cell types (HepG2, MCF-7, MDA-MB-468).
Attained 95.1% accuracy in identifying cytoskeletal perturbations (fixed vs. Cytochalasin B treated cells).

Conclusions:

Multimodal electrical-mechanical flow cytometry offers superior label-free single-cell characterization.
This approach provides a new perspective for accurate intrinsic cell analysis and differentiation.