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The Influence of Permafrost Microorganisms on Monocytes Differentiation In Vitro.
S A Petrov1, Yu G Sukhovei1, L F Kalenova2
1Federal Research Center Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Tyumen, Russia.
Bulletin of Experimental Biology and Medicine
|August 10, 2023
Summary
Microbial metabolites from frozen Bacillus strains modulate human monocyte differentiation. Temperature-dependent metabolites influence monocyte subpopulations, suggesting potential for immune modulation.
Area of Science:
- Microbiology
- Immunology
- Biochemistry
Background:
- Microbial metabolites can influence host immune responses.
- Bacillus species are known for diverse biological activities.
- Monocyte differentiation into subpopulations plays a key role in immunity.
Purpose of the Study:
- To investigate the immunomodulatory effects of metabolites from fossil Bacillus strains on human monocyte differentiation.
- To determine if metabolite production temperature influences their effect on monocyte subpopulations.
Main Methods:
- In vitro culture of human peripheral blood monocytes for 7 days.
- Treatment with metabolites from Bacillus sp. strains 2/09, 9/08, and Bacillus megaterium 8-75 produced at different temperatures (5°C, 22°C, 37°C).
- Flow cytometry analysis to assess monocyte subpopulation changes (classical, intermediate, non-classical).
Main Results:
- Metabolites from all tested Bacillus strains and temperatures weakly reduced classical monocytes (CD14hiCD16-).
- Metabolites stimulated the differentiation of intermediate (CD14+CD16+) and non-classical (CD14loCD16+) monocytes.
- Medium-temperature metabolites of strain 8/75 most effectively promoted intermediate monocyte differentiation, while warm metabolites of strains 8/75 and 2/09 enhanced non-classical monocyte differentiation.
Conclusions:
- Metabolites from specific Bacillus strains, influenced by production temperature, can modulate human monocyte differentiation into distinct subpopulations.
- Bacillus megaterium strain 8/75 and Bacillus sp. strain 2/09 metabolites show promise for further investigation due to their impact on intermediate and non-classical monocytes.
- These findings suggest potential applications in modulating immune responses, particularly in anti-infection strategies.
Keywords:
bacterial metabolitesmonocytes differentiationmonocytes differentiation regulatory factorsmonocytes subpopulationspermafrost fossil microorganismsMore Related Videos
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