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Protocol to locally express cxcl12a during zebrafish olfactory organ development by combining IR-LEGO with live
Marie Zilliox1, Vanessa Tillement2, Thomas Mangeat2
1Unité de biologie Moléculaire, Cellulaire et du Développement (MCD, UMR5077), Centre de Biologie Intégrative (CBI, FR 3743), Université de Toulouse, CNRS, UPS, 118 Route de Narbonne, 31062 Toulouse, France.
Abstract:
Temporal and spatial regulation of gene expression is crucial for proper embryonic development. Infrared laser-evoked gene operator (IR-LEGO) can provide information for various developmental processes. Here, we present a protocol to locally express cxcl12a during zebrafish olfactory organ development1 using a combination of IR-LEGO and live imaging. We describe steps for implementing IR-LEGO, biological sample preparation, live imaging, data collection, and analysis. This protocol can be applied to virtually any genetically modified experimental organism.
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