Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Computed Tomography01:10

Computed Tomography

4.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
4.6K
Positron Emission Tomography01:29

Positron Emission Tomography

4.3K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
4.3K
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

26
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
26
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.4K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.4K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strength-ductility synergy in lightweight aluminium alloys with nano-layered fibres and core-shell nano-particles.

Nature communications·2026
Same author

Alkali-ion-modified zeolitic imidazolate framework glasses.

Nature chemistry·2026
Same author

Structure and composition of grain boundaries and their impact on functional properties of energy materials.

MRS bulletin·2026
Same author

Identifying grain boundary and intragranular pinning centres in Sm<sub>2</sub>(Co,Fe,Cu,Zr)<sub>17</sub> permanent magnets to guide performance optimisation.

Nature communications·2025
Same author

A novel Peltier based low temperature in situ nanoindentation system.

The Review of scientific instruments·2025
Same author

Atomic-scale insights into surface reconstruction and transformation in Co-Cr spinel oxides during the oxygen evolution reaction.

Nature communications·2025
Same journal

Ptychography at all wavelengths.

Nature reviews. Methods primers·2026
Same journal

Droplet-based bioprinting.

Nature reviews. Methods primers·2026
Same journal

Laser capture microdissection.

Nature reviews. Methods primers·2026
Same journal

Extracellular vesicle analysis.

Nature reviews. Methods primers·2026
Same journal

In vivo microelectrode arrays for neuroscience.

Nature reviews. Methods primers·2026
Same journal

Light-based vat-polymerization bioprinting.

Nature reviews. Methods primers·2025
See all related articles

Related Experiment Video

Updated: Jul 16, 2025

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

7.4K

Atom probe tomography.

Baptiste Gault1,2, Ann Chiaramonti3, Oana Cojocaru-Mirédin4

  • 1Max-Planck-Institut für Eisenforschung, Düsseldorf, Germany.

Nature Reviews. Methods Primers
|September 18, 2023
PubMed
Summary
This summary is machine-generated.

Atom probe tomography (APT) offers unparalleled 3D compositional mapping at the atomic scale. This technique provides high sensitivity for all elements, advancing materials science and other fields.

More Related Videos

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.0K
Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

4.9K

Related Experiment Videos

Last Updated: Jul 16, 2025

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

7.4K
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.0K
Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

4.9K

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Surface Science

Background:

  • Atom probe tomography (APT) is a powerful technique for 3D compositional mapping.
  • It offers sub-nanometer resolution and parts-per-million sensitivity for all elements.
  • APT complements existing microscopy and spectroscopy techniques.

Purpose of the Study:

  • To provide an introductory overview of Atom Probe Tomography (APT).
  • To discuss its historical development, recent advancements, and applications.
  • To review APT's performance, limitations, and future directions.

Main Methods:

  • Overview of APT principles and evolution from field ion microscopy.
  • Discussion of specimen preparation techniques, including for nanomaterials.
  • Explanation of data reconstruction and analysis methodologies.

Main Results:

  • APT enables detailed compositional analysis of microstructural features.
  • Applications span geosciences, biological sciences, and materials research.
  • The technique's strengths and limitations are critically reviewed.

Conclusions:

  • APT is a key tool for atomic-scale chemical analysis.
  • Future developments aim for isotopic identification of every atom.
  • Interoperability with other microscopy techniques will enhance its utility.