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Changes in microbial community during hydrolyzed sludge reduction.

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  • 1School of Earth and Environment, Anhui University of Science and Technology, Huainan, China.

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|September 18, 2023
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Summary
This summary is machine-generated.

Enzymes like lysozyme can improve sludge hydrolysis efficiency but may negatively impact wastewater treatment quality. Lysozyme enhanced microbial diversity, offering insights for enzymatic sludge reduction strategies.

Keywords:
community structurediversityhydrolysismicroorganismssludge reduction

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Area of Science:

  • Environmental Science
  • Microbiology
  • Biotechnology

Background:

  • Wastewater treatment generates significant sludge volumes, necessitating efficient sludge reduction methods.
  • Enzymatic hydrolysis is a potential strategy for sludge reduction, but its impact on microbial communities requires further investigation.

Purpose of the Study:

  • To evaluate the effects of lysozyme, α-amylase, and neutral protease on sludge hydrolysis efficiency.
  • To analyze the impact of these enzymes on the microbial community structure and diversity in sequencing batch reactors (SBRs).
  • To assess the influence of enzymatic treatment on effluent quality, including chemical oxygen demand (COD), total nitrogen (TN), and nitrate nitrogen (NO3--N).

Main Methods:

  • Sludge hydrolysis efficiency was measured after treatment with different enzymes.
  • High-throughput sequencing was used to analyze the microbial community composition and diversity.
  • Effluent quality parameters (COD, TN, NO3--N) were monitored.

Main Results:

  • Lysozyme achieved the highest sludge hydrolysis efficiency (48.5%), followed by neutral protease (31%) and α-amylase (22.5%).
  • Enzyme addition, particularly lysozyme, negatively affected effluent quality, decreasing denitrification and phosphorus removal.
  • Lysozyme and α-amylase increased microbial richness and diversity, indicated by smoother abundance grade curves and higher Operational Taxonomic Units (OTUs).
  • The dominant microbial phyla (Proteobacteria, Actinobacteria, Bacteroidetes) remained consistent across treatments.

Conclusions:

  • Enzymatic sludge treatment, especially with lysozyme, can enhance hydrolysis efficiency and microbial diversity.
  • Wastewater treatment performance, including denitrification and phosphorus removal, may be compromised by enzymatic sludge hydrolysis.
  • This study provides valuable data for optimizing enzymatic sludge reduction techniques in practical wastewater treatment operations.