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Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
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PEGylated Dmoc phosphoramidites for sensitive oligodeoxynucleotide synthesis
Komal Chillar1, Yipeng Yin1, Alexander Apostle1
1Department of Chemistry and Health Research Institute, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA. shifang@mtu.edu.
Organic & Biomolecular Chemistry
|November 3, 2023
Abstract:
Sensitive oligodeoxynucleotides (ODNs) can be synthesized using Dmoc phosphoramidites, but only short ODNs were demonstrated. Here, we report the synthesis of much longer ODNs, which was made possible by the use of PEGylated Dmoc (pDmoc) phosphoramidites. The longer ODNs synthesized include those containing the sensitive 4acC epigenetic modification recently discovered in nature.
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