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Protocol to develop force-generating human skeletal muscle organoids.

Mina Shahriyari1, Malte Rinn1, Arne D Hofemeier1

  • 1Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Göttingen, Germany.

STAR Protocols
|December 22, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple method to create human skeletal muscle organoids from pluripotent stem cells. This advance aids in studying muscle development and diseases in vitro.

Keywords:
Cell DifferentiationCell cultureCell-based AssaysOrganoidsTissue Engineering

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Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Developmental Biology

Background:

  • Skeletal muscle models in vitro are crucial for studying muscle function.
  • Existing methods for generating contractile muscle tissue can be complex.

Purpose of the Study:

  • To present a versatile, one-step protocol for generating contractile skeletal muscle organoids (SMOs) from human pluripotent stem cells (PSCs).
  • To enable in vitro studies of human muscle development and related disorders.

Main Methods:

  • Directed differentiation of undifferentiated human PSCs into SMOs.
  • Utilized 3D casting with collagen-I/Matrigel or fibrin/Geltrex hydrogels.
  • Employed various culture platforms for mechanical loading and contractility analysis.

Main Results:

  • Successfully generated functional, contractile skeletal muscle organoids.
  • Demonstrated a versatile and efficient protocol applicable to different hydrogel systems.
  • Established a platform for mechanical loading and contractility assessment.

Conclusions:

  • The described protocol offers a streamlined approach to creating human SMOs.
  • This model is valuable for investigating human skeletal muscle development and congenital muscle disorders.
  • Facilitates in vitro research on muscle physiology and pathology.