Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows
Iago Pereiro1, Anna Fomitcheva-Khartchenko2, Govind V Kaigala3,4
1IBM Research Europe, Rüschlikon, Switzerland. iago.pereiro@roche.com.
Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2024
Abstract
None:
In this chapter, we present the design and fabrication of a device and implementation of a protocol to realize increased efficiency of immunoassays within microtiter plates. The device, WellProbe, is a 3D-structured probe that can be used to deliver precise flows at the bottom of standard well plates to establish concentric areas of shear stress intensities using hydrodynamically confined flows. The protocols involve both operation and data analysis.
