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Related Concept Videos

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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New Poisson denoising method for pulse-count STEM imaging.

Taichi Kusumi1, Shun Katakami1, Ryo Ishikawa2

  • 1Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Chiba 277-8561, Kashiwa, Japan.

Ultramicroscopy
|June 17, 2024
PubMed
Summary
This summary is machine-generated.

We developed a Poisson denoising method for electron counting imaging in scanning transmission electron microscopy. This method significantly reduces electron dose while improving image quality for atomic-resolution studies.

Keywords:
Bayesian inferenceMarkov random field modelPoisson denoisingPulse countScanning transmission electron microscopy

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Area of Science:

  • Materials Science
  • Physics
  • Imaging Science

Background:

  • Advancements in electron microscopy detectors enable direct electron counting per pixel.
  • Electron counting imaging offers potential for higher resolution and reduced sample damage.
  • Optimizing denoising is crucial for extracting meaningful data from low-dose electron counting images.

Purpose of the Study:

  • To develop and optimize a denoising method specifically for atomic-resolution scanning transmission electron microscopy (STEM) electron counting images.
  • To enable significant reduction in electron dose while maintaining or improving image quality.
  • To compare reconstruction methods for denoised electron counting images.

Main Methods:

  • Proposed a Poisson denoising method based on Markov random field models and Bayesian inference.
  • Applied the method to atomic-resolution scanning transmission electron microscopy images.
  • Investigated reconstruction from multiple non-integrated images versus integrated images.

Main Results:

  • The proposed Poisson denoising method effectively reduces noise in electron counting images.
  • Achieved significant electron dose reduction, by a factor of approximately 15 times or more.
  • Reconstruction from multiple, non-integrated images yielded superior results compared to using a single integrated image.

Conclusions:

  • The developed Poisson denoising method is effective for low-dose, atomic-resolution STEM electron counting imaging.
  • Significant dose reduction is achievable, preserving structural information.
  • Multi-image reconstruction without integration is a more effective strategy for this type of imaging.