Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods to Assess Microbial Populations

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>RBX1</i> loss sensitizes tubo-ovarian, high-grade serous ovarian cells to CDK2 inhibition by SNS-032.

Frontiers in cell and developmental biology·2026

Same author

Patient-derived organoids reveal hypoxia-driven plasticity and therapeutic vulnerabilities in pheochromocytomas and paragangliomas.

bioRxiv : the preprint server for biology·2025

Same author

<i>CHEK1</i> is a synthetic lethal interactor of <i>FBXO7</i> in colonic epithelial cells.

Molecular therapy. Oncology·2025

Same author

Loss of EMI1 compromises chromosome stability and is associated with cellular transformation in colonic epithelial cell contexts.

British journal of cancer·2024

Same author

PROTACs: Current and Future Potential as a Precision Medicine Strategy to Combat Cancer.

Molecular cancer therapeutics·2024

Same author

Survey of NF1 inactivation by surrogate immunohistochemistry in ovarian carcinomas.

Gynecologic oncology·2023

Search research articles

Related Experiment Video

Updated: May 25, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Evaluating Chromosome Instability and Genotoxicity Through Single Cell Quantitative Imaging Microscopy.

Rubi Campos Gudiño^1,2, Kailee A Rutherford^1,2, Kirk J McManus^3,4

¹Paul Albrechtsen Research Institute, CancerCare Manitoba, Winnipeg, MB, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|June 24, 2024

Summary

This study introduces a rapid quantitative imaging microscopy (QuantIM) method to assess chromosome instability (CIN) and genotoxicity. The QuantIM approach aids in identifying new CIN genes and genotoxic agents for cancer research.

Keywords:

Cancer Chromosome instability Genome instability Genotoxicity Micronucleus/micronuclei Nuclear area Quantitative imaging microscopy

More Related Videos

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

Related Experiment Videos

Last Updated: May 25, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry

Published on: May 13, 2019

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

Area of Science:

Genetics
Cell Biology
Cancer Research

Background:

Genome stability is crucial for eukaryotic cell function and survival.
Chromosome instability (CIN) is a hallmark of cancer, driving genetic heterogeneity.
Micronuclei and nuclear area changes are established markers for genotoxicity and CIN.

Purpose of the Study:

To present a facile quantitative imaging microscopy (QuantIM) approach for rapid assessment of CIN phenotypes and genotoxicity.
To provide optimized protocols, imaging settings, and data analysis strategies for robust CIN and genotoxicity assays.
To enable large-scale screening for novel CIN genes and genotoxic compounds.

Main Methods:

Development and optimization of quantitative imaging microscopy (QuantIM) protocols.
Simultaneous assessment of nuclear area changes and micronucleus formation in single cells.
Application of statistical analyses and data visualization for robust data interpretation.

Main Results:

A facile QuantIM method was established for rapid quantification of CIN phenotypes.
The approach allows for simultaneous assessment of CIN markers and genotoxicity.
Optimized protocols ensure high-quality data for diverse experimental and clinical applications.

Conclusions:

The presented QuantIM approach facilitates efficient identification of novel CIN genes.
This method aids in discovering new genotoxic agents relevant to cancer development.
QuantIM provides deeper insights into aberrant genes and pathways underlying CIN and genotoxicity.