Related Experiment Video
Updated: Jun 27, 2026
10:01
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
7.6K
Heterogeneous Integration of Acoustic Microextraction with an Optoelectronic Sensor on Glass for Nucleic Acid Testing
Zhiwei Li1, Tiechuan Li1, Francesca Costantini2
1State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, Tianjin 300072, China.
Analytical Chemistry
|July 6, 2024
Summary
This study introduces a novel lab-on-a-chip (LOC) for nucleic acid testing (NAT) using acoustic streaming. The integrated system simplifies sample preparation, enabling rapid and sensitive on-site detection of genetic mutations.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Molecular Diagnostics
Background:
- Lab-on-a-chip (LOC) systems offer high sensitivity and portability for on-site testing.
- Full integration of sample preparation, purification, and multiplexed detection in LOCs remains a significant challenge.
- Existing LOCs often require complex peripherals and extensive sample processing.
Purpose of the Study:
- To develop a novel, fully integrated lab-on-a-chip system for nucleic acid testing (NAT).
- To utilize acoustic streaming for simplified and efficient nucleic acid extraction.
- To enable compact, hand-held NAT instruments for on-site genetic analysis.
Main Methods:
- Heterogeneous integration of complementary metal oxide semiconductor (CMOS)-compatible devices (acoustic resonator, resistors, sensors) into a LOC.
- Application of programmed acoustic streaming for particle and fluid manipulation, including cell lysis, nucleic acid capture, and elution.
- Microelectromechanical system (MEMS) approach to avoid conventional mechanical actions and complex flow control.
Main Results:
- Achieved approximately 90% nucleic acid (NA) extraction efficiency within 5 minutes.
- Demonstrated a limit of detection (LOD) of 1 copy/μL for target NA in plasma.
- Validated the system's robustness using plasma samples with epidermal growth factor receptor (EGFR) gene mutations.
Conclusions:
- The developed LOC system successfully integrates sample preparation and detection for NAT.
- Acoustic streaming significantly simplifies the nucleic acid extraction process, enabling rapid on-site testing.
- The compact, hand-held instrument demonstrates potential for widespread application in point-of-care diagnostics.
