Magnetically Responsive Enzyme and Hydrogen-Bonded Organic Framework Biocomposites for Biosensing

Area of Science:

• Materials Science
• Biotechnology
• Nanotechnology

Background:

• Hydrogen-bonded organic frameworks (HOFs) are emerging porous materials with potential in biocatalysis.
• Enzyme immobilization is crucial for enhancing enzyme stability and reusability.
• Magnetic nanoparticles (MNPs) offer advantages for catalyst separation and manipulation.

Purpose of the Study:

• To develop a one-pot synthesis for multicomponent HOF biocomposites co-immobilizing enzymes and MNPs.
• To investigate the dynamic localization and catalytic properties of the resulting biocatalysts.
• To demonstrate the application of these magnetic biocatalysts in a microfluidic biosensor.

Main Methods:

• One-pot synthesis of HOFs incorporating enzymes and MNPs.
• Characterization of the magnetic biocatalysts (MNPs-enzyme@BioHOF-1).
• Evaluation of enzyme activity retention (Catalase and Glucose Oxidase).
• Fabrication and testing of a glucose microfluidic biosensor using MNPs-GOx@BioHOF-1.

Main Results:

• Successful co-immobilization of enzymes and MNPs into HOF crystals.
• MNPs-enzyme@BioHOF-1 biocatalysts exhibited dynamic localization controllable by a permanent magnet.
• Co-immobilization with MNPs enhanced the activity retention of Catalase and Glucose Oxidase.
• A proof-of-concept glucose microfluidic biosensor was successfully developed, utilizing magnetic positioning of the biocomposite.

Conclusions:

• The developed magnetic HOF biocomposites provide a versatile platform for enzyme immobilization and biocatalysis.
• The dynamic localization property enabled by MNPs facilitates catalyst recovery and integration into microfluidic devices.
• This work opens new avenues for the application of HOF biocomposites in advanced sensing and catalytic systems.