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Super-resolution Fluorescence Microscopy

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Related Experiment Video

Updated: Jun 5, 2025

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Simultaneous Dual-region Functional Imaging in Miniaturized Two-photon Microscopy.

Zixiao Zhang¹, Shing-Jiuan Liu¹, Ben Mattison^2,3

¹Department of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.

Biomedical Optics (Washington, D.C.)

|December 5, 2024

Summary

This summary is machine-generated.

This study presents a new two-photon microscope for faster brain imaging in mice. The technology enables simultaneous dual-region in-vivo imaging, improving neuroscience research capabilities.

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Last Updated: Jun 5, 2025

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

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Published on: December 12, 2012

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Published on: March 13, 2016

Area of Science:

Neuroscience
Biomedical Engineering
Optical Imaging

Background:

Simultaneous imaging of multiple brain regions is crucial for understanding complex neural circuits.
Existing two-photon microscopy techniques face limitations in speed and spatial coverage.
Developing advanced imaging platforms is essential for high-resolution in-vivo neuroscience research.

Purpose of the Study:

To demonstrate a novel miniaturized spatial-multiplexed two-photon microscope for enhanced in-vivo brain activity imaging.
To achieve simultaneous imaging of two distinct regions in the mouse cortex.
To increase the imaging speed and efficiency for neural signal acquisition.

Main Methods:

Development of a miniaturized spatial-multiplexed two-photon microscope platform.
In-vivo imaging of brain activity in the mouse cortex across two selected regions.
Application of computational methods for demixing and extracting neuronal signals from dual imaging regions.

Main Results:

Successful simultaneous dual-region in-vivo imaging of brain activity in the mouse cortex.
Demonstration of doubled imaging speed compared to conventional methods.
Effective computational demixing and extraction of neuronal signals from the two imaged regions.

Conclusions:

The developed spatial-multiplexed two-photon microscope enables faster and more efficient dual-region brain imaging in vivo.
This technology offers a significant advancement for studying neural dynamics in distributed brain areas.
The platform provides a valuable tool for future neuroscience investigations requiring high-throughput neural activity monitoring.