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Related Experiment Video

Updated: Jun 5, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

Time-Multiplexed Miniaturized Two-Photon Microscopy.

Shing-Jiuan Liu¹, Zixiao Zhang¹, Ben Mattison^2,3

¹Department of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.

Biomedical Optics (Washington, D.C.)

|December 5, 2024

Summary

This summary is machine-generated.

We developed a faster, miniaturized two-photon microscope (TM-MINI2P) for high-speed in-vivo calcium imaging in mouse brains. This advancement doubles imaging speed without sacrificing spatial resolution.

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Last Updated: Jun 5, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

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In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

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Published on: July 11, 2019

Area of Science:

Neuroscience
Biomedical Engineering
Optical Imaging

Background:

High-speed in-vivo imaging is crucial for understanding neural dynamics.
Existing two-photon microscopy techniques face limitations in speed or resolution.
Miniaturization is key for advanced in-vivo neuroscience applications.

Purpose of the Study:

To introduce a novel time-multiplexed miniaturized two-photon microscope (TM-MINI2P).
To demonstrate TM-MINI2P's capability for high-speed, high-resolution in-vivo imaging.
To validate its application in mouse cortical circuit analysis.

Main Methods:

Development of a time-multiplexed miniaturized two-photon microscope (TM-MINI2P).
Implementation of advanced optical designs for enhanced speed.
In-vivo calcium imaging experiments in the mouse cortex.

Main Results:

Achieved a two-fold increase in imaging speed compared to conventional methods.
Maintained high spatial resolution during high-speed imaging.
Successfully captured dynamic neural activity in the mouse brain.

Conclusions:

TM-MINI2P offers a significant advancement for high-speed in-vivo neural imaging.
The technology enables deeper insights into brain function at faster timescales.
Miniaturized two-photon microscopy is a promising tool for future neuroscience research.