Profiling of Biofluid Metabolites with a Kinetically Differentiated Binary Biosensing Platform.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biochemistry

Background:

• Monitoring biofluid metabolites is crucial for disease diagnosis and health management.
• Existing methods for metabolite detection often have limitations in range and universality.
• Nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] is a key cofactor involved in numerous metabolic processes.

Purpose of the Study:

• To develop a universal biosensing platform for profiling diverse biofluid metabolites.
• To overcome the limitations of traditional unitary detection strategies for NAD(P)H.
• To establish a method for accurate and expanded range quantification of metabolites.

Main Methods:

• Development of a kinetically differentiated binary biosensing platform using cyanine and quinolinium derivative dyes.
• Utilizing the platform to measure variations in NAD(P)H concentration during biochemical reactions.
• Quantification of serum metabolites including sorbitol, 2-hydroxybutyric acid (2HB), and α-ketoglutarate (AKG).
• Construction of a paper-based assay for point-of-care (POC) metabolite profiling.

Main Results:

• The binary biosensing platform demonstrated a 20-fold wider linear range compared to traditional methods.
• Accurate quantification of serum sorbitol, 2HB, and AKG with accuracies exceeding 93%.
• Successful development of a paper-based assay for POC applications.
• The platform showed versatility for analyzing molecules reacting with NAD(P)H.

Conclusions:

• The kinetically differentiated binary biosensing platform offers a universal and expanded-range approach for metabolite profiling.
• This technology provides a promising paradigm for high-throughput analysis of disease-associated biomolecules.
• The developed platform holds significant potential for advancing disease diagnosis and health management through accessible metabolite monitoring.