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Updated: May 8, 2025
Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
Enhancing Thermodynamic and Kinetic Performance of Microfluidic Interface-Based Circulating Fetal Cell Isolation for
Juan Song1, Yue Zheng2, Xiaodan Huang1
1Discipline of Intelligent Instrument and Equipment, College of Chemistry and Chemical Engineering, Molecular Diagnostic Laboratory for Precision Medicine, the First Affiliated Hospital of Xiamen University, Xiang'an Hospital, Women and Children's Hospital, School of Medicine, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361005, China.
We developed a novel magnetic microfluidic chip using aptamer-nanobody technology to improve cell capture efficiency. This AMP-chip enhances both speed and strength of binding for precise isolation of target cells.
Area of Science:
- Biotechnology and Biomedical Engineering
- Microfluidics and Lab-on-a-Chip Technologies
- Molecular Recognition and Affinity Assays
Background:
- Multivalent strategies are used in microfluidics to improve cell capture, but balancing binding kinetics and thermodynamics is difficult.
- Existing methods face challenges in achieving optimal binding affinity and speed simultaneously for efficient target cell isolation.
Purpose of the Study:
- To introduce a synergistic Aptamer-nanobody hetero-Multivalency Programmable magnetic fluid microfluidic chip (AMP-chip).
- To enhance both thermodynamic and kinetic properties of the capture interface by integrating ligands with distinct binding characteristics.
- To improve the isolation of circulating nucleated red blood cells (cNRBCs) from peripheral blood mononuclear cells (PBMCs).
Main Methods:
- Integration of two distinct, non-interfering recognition molecules (high-affinity nanobody and rapid-binding aptamer) onto nanomagnetic beads.
- Utilizing CD71 receptor recognition targets for cell binding.
- Incorporation of a herringbone microarray within the AMP-chip to increase cell-ligand interaction surface area.
Main Results:
- The AMP-chip demonstrated enhanced binding kinetics and thermodynamic stability through intermolecular complementarity.
- Achieved rapid and tight recognition of CD71 receptor on target cells by leveraging the synergistic effects of aptamer and nanobody.
- Significantly improved sensitivity and accuracy in isolating cNRBCs from PBMCs, with nondestructive cell release and preserved cell viability.
Conclusions:
- The developed AMP-chip presents a novel thermodynamic-kinetic synergistic heteromultivalency interface.
- This approach offers a promising platform for efficient and non-damaging isolation of target cells.
- The technology holds significant potential for various clinical applications, particularly in sensitive cell analysis.
