Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

262
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
262
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

241
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
241
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.0K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revealing global stoichiometry conservation architecture in cells from Raman spectral patterns.

eLife·2026
Same author

Observation of persister cell histories reveals diverse modes of survival in antibiotic persistence.

eLife·2025
Same author

A unified framework for measuring selection on cellular lineages and traits.

eLife·2022
Same author

Single mutation makes Escherichia coli an insect mutualist.

Nature microbiology·2022
Same author

History-dependent physiological adaptation to lethal genetic modification under antibiotic exposure.

eLife·2022
Same author

Intrinsic growth heterogeneity of mouse leukemia cells underlies differential susceptibility to a growth-inhibiting anticancer drug.

PloS one·2021
Same journal

Vogel spiral-based tilt-scan averaging approach for robust and efficient diffraction contrast suppression in DPC STEM.

Microscopy (Oxford, England)·2026
Same journal

Development of a specialized diamond knife for controlled notch introduction in ultrathin polymer films for in situ tensile transmission electron microscopy.

Microscopy (Oxford, England)·2026
Same journal

Study of nanocrystals within lamellar structures of polyvinylidene fluoride using phase plate scanning transmission electron microscopy.

Microscopy (Oxford, England)·2026
Same journal

Capability of angle-resolved SXES experiment examined by hexagonal BN and its application for the chemical bonding state of Fe2B.

Microscopy (Oxford, England)·2026
Same journal

Cryo-EELS elemental mapping of organic-solvent systems.

Microscopy (Oxford, England)·2026
Same journal

In-situ biasing DPC STEM observation of GaAs p-n junction.

Microscopy (Oxford, England)·2026
See all related articles

Related Experiment Video

Updated: May 10, 2025

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

16.9K

Live-cell omics with Raman spectroscopy.

Ken-Ichiro F Kamei1, Yuichi Wakamoto1,2,3,4

  • 1Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.

Microscopy (Oxford, England)
|April 24, 2025
PubMed
Summary
This summary is machine-generated.

Single-cell Raman spectroscopy enables non-destructive, genome-wide molecular profiling of living cells. This advance allows tracking cellular phenotype and omics profile dynamics, crucial for understanding cell differentiation and adaptation.

Keywords:
Raman spectroscopycellular plasticitylive-cell omicssingle-cell analysis

More Related Videos

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.3K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.5K

Related Experiment Videos

Last Updated: May 10, 2025

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

16.9K
An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

9.3K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Spectroscopy

Background:

  • Genome-wide profiling (e.g., transcriptomics, proteomics) is vital for understanding cellular behavior and phenotypic plasticity.
  • Simultaneous characterization of single-cell phenotypes and omics profiles is essential for studying dynamic biological processes.
  • Current in situ omics profiling methods are often destructive, limiting the study of post-measurement cellular dynamics.

Purpose of the Study:

  • To review recent advancements in imaging-based omics profiling techniques.
  • To present a method for inferring genome-wide omics profiles from single-cell Raman spectra.
  • To highlight the potential of Raman spectroscopy for live-cell omics studies.

Main Methods:

  • Overview of current imaging-based omics profiling technologies.
  • Detailed explanation of inferring omics profiles from single-cell Raman spectra.
  • Utilizing Raman spectroscopy for non-destructive, non-staining molecular analysis of living cells.

Main Results:

  • Raman spectroscopy allows for the characterization of genome-wide molecular profiles in living cells.
  • The method is non-destructive and does not require cell staining, preserving cell viability.
  • This approach enables the study of cellular dynamics and omics profile changes over time.

Conclusions:

  • Single-cell Raman spectroscopy offers a promising avenue for live-cell omics research.
  • This technique facilitates the simultaneous observation of cellular phenotypes and molecular profiles.
  • It has the potential to significantly advance the understanding of dynamic cellular processes like differentiation and adaptation.