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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

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In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
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Factors Influencing Drug Absorption: Pharmaceutical Parameters01:28

Factors Influencing Drug Absorption: Pharmaceutical Parameters

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Solid dosage forms such as tablets and capsules undergo rigorous manufacturing processes to ensure stability and effectiveness. Their dissolution and absorption properties are influenced significantly by the choice of excipients (inactive ingredients that serve various roles in the formulation), and the methodology applied during production. The manufacturing parameters, such as compression force and granulation techniques, significantly affect dissolution rates. Elevated compression forces...
197
Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH01:21

Factors Affecting Dissolution: Drug pKa, Lipophilicity and GI pH

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Drug absorption within the gastrointestinal (GI) tract is a complex process influenced by several critical factors, including the site pH, the drug's dissociation constant (pKa), and the drug's lipophilicity. The GI tract exhibits a pH gradient, with an acidic environment in the stomach and a more alkaline environment in the small intestine. This pH variation directly affects the ionization state of drugs.
A drug's pKa and the pH of the gastrointestinal (GI) tract play crucial roles...
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In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
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Preparing O/W/O Emulsion for Curcumin (Curcuma longa) Delivery and In Vitro Digestibility Assay.

Kristýna Opustilová1, Barbora Lapčíková1, Daniela Sumczynski1

  • 1Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlin, Nam. T. G. Masaryka 5555, 760 01 Zlin, Czech Republic.

International Journal of Molecular Sciences
|June 26, 2025
PubMed
Summary

Multiple emulsions offer superior curcumin delivery compared to simple emulsions, showing enhanced stability and digestibility. This research highlights their potential for improved nutrient bioavailability.

Keywords:
curcuma root powderscurcumindigestibilityemulsionsencapsulationparticle sizestability

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Area of Science:

  • Food Science and Technology
  • Colloid and Surface Chemistry
  • Nutritional Science

Background:

  • Curcumin, a potent bioactive compound, suffers from poor bioavailability.
  • Emulsion-based delivery systems are explored to enhance curcumin's stability and absorption.
  • Understanding the structural and digestive properties of different emulsion types is crucial for optimizing delivery.

Purpose of the Study:

  • To evaluate simple oil-in-water (O/W) and multiple oil-in-water-in-oil (O/W/O) emulsions as carriers for curcumin delivery.
  • To compare the stability, particle size, rheological properties, and in vitro digestibility of O/W and O/W/O emulsions containing curcumin.
  • To assess the in vitro digestibility of curcumin from emulsions versus fresh and dried curcuma root powders.

Main Methods:

  • Emulsion characterization using Differential Scanning Calorimetry (DSC), Dynamic Light Scattering (DLS), and rheological analysis.
  • Determination of freezable water content (Wfs) in both emulsion types.
  • In vitro digestibility studies of curcumin in emulsions and curcuma root powders monitored by High-Performance Liquid Chromatography (HPLC).

Main Results:

  • O/W/O emulsions exhibited significantly lower freezable water (23.7%) compared to O/W emulsions (80.4%).
  • Multiple O/W/O emulsions demonstrated greater stability and a larger mean surface diameter (approx. 2x) than simple O/W emulsions.
  • O/W/O emulsions were superior for curcumin delivery, achieving 50.6% digestibility in simulated stomach and intestinal conditions, outperforming dried curcuma powders (52.9-78.8% DMD) but not fresh curcuma (95.5% DMD).

Conclusions:

  • Multiple O/W/O emulsions provide a more stable and effective matrix for curcumin delivery compared to simple O/W emulsions.
  • The complex structure of multiple emulsions enhances curcumin's stability and improves its in vitro digestibility.
  • This study supports the potential of multiple emulsions as a promising strategy to enhance the bioavailability of curcumin.