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A Promising Point-of-Care Testing Strategy: Ultrasmooth Gold Nanogroove Arrays Biosensor Combined with Initial Rate
1State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials Science and Engineering, Sun Yat-Sen University, No.132, East Outer Ring Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong, 510006, China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 22, 2025
Summary
This study introduces a novel plasmonic biosensor using ultrasmooth gold nanogroove arrays (UGNA) and initial rate analysis (IRA) for rapid, ultrasensitive protein detection. The integrated system shows significant potential for point-of-care testing (POCT) devices.
Area of Science:
- Plasmonics
- Biosensing
- Nanotechnology
Background:
- Plasmonic biosensors offer high sensitivity but face challenges in mass production, reproducibility, and integration for point-of-care testing (POCT).
- Existing research tools require optimization for commercial applications demanding rapid, ultrasensitive, and reproducible detection.
Purpose of the Study:
- To develop a label-free, ultrasensitive, and rapid assay protocol for quantitative protein detection in serum samples.
- To integrate ultrasmooth gold nanogroove arrays (UGNA) biosensor with initial rate analysis (IRA) for enhanced performance.
- To assess the potential of the developed system for point-of-care testing (POCT) applications.
Main Methods:
- Fabrication of ultrasmooth gold nanogroove arrays (UGNA) using a high-reproducibility template-stripping technique.
- Integration of UGNA biosensor with initial rate analysis (IRA) for kinetic analysis.
- Utilized a miscellaneous-protein-mixed scheme to accelerate analyte binding kinetics through surface passivation.
Main Results:
- Achieved a wide linear range of AFP detection (1-10^4 ng/mL) with a 160-fold enhancement in detection time (approx. 70 s) for ultralow concentrations.
- UGNA demonstrated a high surface figure of merit (FOMsurf) under normal incidence, enabling miniaturization and multiplexing.
- Template-stripping fabrication ensures high reproducibility and potential for low-cost mass production of UGNA biosensors.
Conclusions:
- The integrated UGNA biosensor and IRA method provide a label-free, ultrasensitive, and rapid platform for protein detection in serum.
- The system's high performance, reproducibility, and potential for mass production make it suitable for point-of-care testing (POCT) devices.
- This biosensing approach significantly advances the field towards practical, commercialized diagnostic tools.
