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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Related Experiment Video

Updated: Sep 8, 2025

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models

Published on: July 24, 2019

Fluorescein Angiography.

Andrea Andrade¹, Stephen H Tsang², Tarun Sharma³

¹Universidade Federal de Pernambuco, Recife, Brazil.

Advances in Experimental Medicine and Biology

|July 30, 2025

Summary

This summary is machine-generated.

Fluorescein angiography (FA) uses injected fluorescein dye to image retinal and choroidal circulation. This luminescence technique differs from autofluorescence, which detects naturally occurring molecules.

Keywords:

Angiography Fluorescein

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Last Updated: Sep 8, 2025

Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in Rat and Rabbit Aneurysm Models

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Published on: July 24, 2019

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

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Published on: March 11, 2016

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

Area of Science:

Ophthalmology
Medical Imaging
Biophysics

Background:

Fluorescein angiography (FA), or Intravenous Fluorescein Angiography (IVFA), is a key imaging technique.
It visualizes the retina and choroid's blood flow.
The technique relies on luminescence principles.

Purpose of the Study:

To explain the physical principles behind fluorescein angiography.
To differentiate FA from other luminescence imaging techniques like autofluorescence.

Main Methods:

Describes the excitation and emission of light by molecules.
Explains the use of exogenous molecules in FA.
Contrasts this with endogenous molecules used in autofluorescence.

Main Results:

FA utilizes exogenous molecules for imaging.
Autofluorescence uses endogenous molecules.
Both are forms of luminescence imaging.

Conclusions:

Fluorescein angiography is a luminescence-based imaging method.
The source of the excited molecule (exogenous vs. endogenous) defines the technique.
Understanding these principles is crucial for interpreting ocular imaging.