Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

11.3K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.3K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

496
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
496
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.5K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.5K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

6.4K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
6.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

14.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
14.1K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of drying method on the surface properties of cellulose nanofibril films.

Scientific reports·2026
Same author

Noninvasive imaging techniques to map language areas using BOLD signal fluctuations in pediatric epilepsy: a review.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2026
Same author

Sleep, internalizing symptoms, and health-related quality of life in children with neurodevelopmental disorders: a cross-sectional analysis of cohort data from three research programs in Canada.

Frontiers in sleep·2025
Same author

Retinal Histology and Anatomical Landmarks.

Advances in experimental medicine and biology·2025
Same author

Fluorescein Angiography.

Advances in experimental medicine and biology·2025
Same author

Looking beyond epilepsy management: The impact of mental health, quality of life, and transition-readiness on transition-aged adolescents with epilepsy and varying cognitive abilities.

Epilepsy & behavior : E&B·2025
Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026
Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026
Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026
Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026
Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Sep 8, 2025

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

11.5K

Fundus Autofluorescence.

Andrea Andrade1, Jorge Pincay2, Promie Faruque3

  • 1Universidade Federal de Pernambuco, Recife, Brazil.

Advances in Experimental Medicine and Biology
|July 30, 2025
PubMed
Summary
This summary is machine-generated.

Fundus autofluorescence (FAF) imaging uses light to visualize natural fluorescence in the eye. This rapid, noninvasive technique helps in understanding eye conditions by analyzing emitted light patterns.

Keywords:
AutofluorescenceFundusImaging

More Related Videos

Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

4.5K
Autofluorescence Imaging to Evaluate Red Algae Physiology
05:54

Autofluorescence Imaging to Evaluate Red Algae Physiology

Published on: February 17, 2023

1.5K

Related Experiment Videos

Last Updated: Sep 8, 2025

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

11.5K
Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

4.5K
Autofluorescence Imaging to Evaluate Red Algae Physiology
05:54

Autofluorescence Imaging to Evaluate Red Algae Physiology

Published on: February 17, 2023

1.5K

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Biophotonics

Background:

  • Fundus autofluorescence (FAF) imaging is a key noninvasive diagnostic tool.
  • It visualizes naturally occurring fluorophores within the ocular fundus.
  • Understanding FAF principles is crucial for interpreting retinal health.

Purpose of the Study:

  • To explain the fundamental physical principles of FAF imaging.
  • To highlight its noninvasive and rapid nature for clinical application.
  • To establish the basis for FAF's role in visualizing ocular fluorophores.

Main Methods:

  • Describes the physical principle of FAF: excitation and emission of light.
  • Details the process of using specific wavelengths for excitation.
  • Explains the detection of longer wavelength emissions.

Main Results:

  • FAF imaging allows visualization of fluorophores in the fundus.
  • The technique relies on light excitation and subsequent emission.
  • The emitted light provides insights into retinal biochemistry.

Conclusions:

  • FAF imaging is a rapid and noninvasive method for visualizing ocular fluorophores.
  • Its physical basis involves light excitation and emission principles.
  • FAF serves as a valuable tool in ophthalmological diagnostics.