An Integrated System Combining Filter-Assisted Sample Preparation and Colorimetric Biosensing for Rapid Pathogen...

Area of Science:

• Food Science
• Microbiology
• Analytical Chemistry

Background:

• Climate change elevates microbial contamination risks in food.
• Food residues interfere with direct biosensor detection of pathogens.
• Real-time biosensors are crucial for food safety.

Purpose of the Study:

• To develop an integrated system for rapid, on-site detection of foodborne pathogens in complex food matrices.
• To overcome limitations posed by food residues in biosensor analysis.
• To ensure accuracy and stability in pathogen detection.

Main Methods:

• An integrated system combining filter-assisted sample preparation (FASP) and an immunoassay-based colorimetric biosensor.
• FASP preprocesses samples to separate bacteria from food residues.
• The system was validated on various food matrices (vegetables, meats, cheese brine).

Main Results:

• Achieved a detection limit of 10^1 CFU/mL for key pathogens (E. coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes).
• Sample preparation was rapid (<3 min), with detection completed within 2 hours.
• Demonstrated broad applicability across diverse food types with minimal specialized equipment.

Conclusions:

• The FASP-biosensor system enables rapid, simple, and accurate on-site detection of foodborne pathogens.
• This approach enhances food safety by providing real-time, rapid-response capabilities.
• The system is suitable for various food matrices, addressing critical needs in food surveillance.