Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Blood Biomarker Detection Using Integrated Microfluidics with Optical Label-Free Biosensor.

Sensors (Basel, Switzerland)·2024

Same author

Handheld Biosensor System Based on a Gradient Grating Period Guided-Mode Resonance Device.

Biosensors·2024

Same author

Gradient Guided-Mode Resonance Biosensor with Smartphone Readout.

Biosensors·2023

Same author

A Hierarchical Hand Gesture Recognition Framework for Sports Referee Training-Based EMG and Accelerometer Sensors.

IEEE transactions on cybernetics·2020

Search research articles

Related Experiment Video

Updated: Jul 28, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Handheld Lab-on-a-Chip System for Label-Free Dual-Plex Detection of Biomarkers Through On-Chip Plasma Separation.

Chen-Yuan Chang¹, Yuan-Pei Lei¹, Chien Chieh Chiang¹

¹Department of Mechanical Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan.

|November 26, 2025

Summary

This study presents a handheld lab-on-a-chip platform for rapid biomarker detection in whole blood. The device enables sensitive, real-time analysis, advancing point-of-care diagnostics.

Keywords:

guided-mode resonance immunosensor lab-on-a-chip label-free biosensor

More Related Videos

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Related Experiment Videos

Last Updated: Jul 28, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Analytical Chemistry
Point-of-Care Diagnostics

Background:

Effective disease diagnosis relies on rapid biomarker detection in complex biological fluids like whole blood, especially in point-of-care settings.
Current methods often face challenges in speed, sensitivity, and suitability for decentralized use.

Purpose of the Study:

To develop a handheld lab-on-a-chip (LOC) platform for integrated plasma separation and label-free, dual-plex biomarker detection.
To demonstrate the platform's capability for sensitive, specific, and reproducible detection of biomarkers in whole blood.

Main Methods:

Development of a two-stage filtration unit for efficient on-chip plasma separation from whole blood.
Integration of a novel gradient grating period guided-mode resonance sensor array for multiplexed, label-free biosensing.
Utilization of a compact handheld reader for signal acquisition and analysis.

Main Results:

Successful demonstration of sensitive and specific detection of model biomarkers (albumin and creatinine) in plasma and whole blood.
Achieved low detection limits: 0.8 μg/mL for albumin and 1.44 μg/mL for creatinine.
Exhibited minimal nonspecific binding, confirming the platform's specificity and reliability.

Conclusions:

The developed handheld LOC platform offers a robust and accessible solution for decentralized diagnostics.
The integrated system enables real-time, dual-plex biomarker detection with high sensitivity and specificity.
This technology holds significant potential for improving disease monitoring and diagnosis in point-of-care environments.