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Related Experiment Video

Updated: Feb 22, 2026

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Sperm DNA Fragmentation: The Concept and Its Use in Assisted Reproduction.

Jaime Gosálvez¹, Carmen López Fernández¹, Javier Bartolomé-Nebreda^1,2

¹Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Advances in Experimental Medicine and Biology

|February 20, 2026

Summary

This summary is machine-generated.

Sperm DNA fragmentation (SDF) impacts fertility and assisted reproduction outcomes in humans and animals. Managing SDF is crucial for improving reproductive success, especially in endangered species.

Keywords:

Animal reproduction Assisted reproduction Male factor Sperm Sperm DNA fragmentation

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Area of Science:

Reproductive Biology
Genetics
Conservation Biology

Background:

Sperm DNA quality is declining globally in humans and animals.
Sperm DNA fragmentation (SDF) negatively affects reproductive outcomes.
Assisted reproduction techniques require optimal sperm DNA integrity.

Purpose of the Study:

To review the causes and consequences of SDF in humans and animals.
To explore methods for assessing and managing SDF.
To apply findings to enhance assisted reproduction strategies, particularly for endangered species.

Main Methods:

Review of scientific literature on sperm DNA damage and repair mechanisms.
Analysis of factors contributing to SDF, including biological and iatrogenic causes.
Examination of methodologies for SDF assessment and mitigation strategies.

Main Results:

SDF assessment is a critical indicator of semen quality, especially for intracytoplasmic sperm injection (ICSI).
Causes of high SDF are multifactorial, requiring standardized protocols for management.
Personalized treatments are essential for optimizing gamete quality in humans and tailoring strategies for endangered species.

Conclusions:

Effective management of SDF is vital for successful assisted reproduction in humans and conservation efforts for endangered species.
Tailored strategies, mimicking natural selection, can improve sperm DNA quality and fertilization efficacy.
Further research into synergistic effects of management strategies can enhance sperm availability for fertilization.