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Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters
Shuai Wang1, Haiping Sun1, Xiyu Lv2
1School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
Ultrasonics Sonochemistry
|March 22, 2026
Summary
Stir-frying Fructus Tribuli (FT) enhances its polysaccharides (SFP), improving gut health and reducing hypertension more effectively than unheated FT polysaccharides (FP). This processing method remodels bioactive molecules for amplified therapeutic benefits.
Area of Science:
- Pharmacology and Traditional Chinese Medicine
- Natural Product Chemistry
- Gastroenterology and Cardiovascular Research
Background:
- Fructus Tribuli (FT) is known for antihypertensive effects, potentially linked to its polysaccharides.
- Traditional processing, like stir-frying, may enhance FT's therapeutic properties.
- Limited research exists on the specific effects of FT polysaccharides and processing on health outcomes.
Purpose of the Study:
- To optimize ultrasound-assisted extraction (UAE) of FT polysaccharides (FP).
- To compare the structural and antihypertensive effects of FP and stir-fried FT polysaccharides (SFP).
- To elucidate the mechanisms underlying the enhanced efficacy of SFP in spontaneously hypertensive rats.
Main Methods:
- Optimized UAE for FP yield using response surface methodology.
- Isolated and characterized FP and SFP, comparing their structures.
- Evaluated antihypertensive and vascular protective effects in spontaneously hypertensive rats, including gut microbiota and intestinal barrier function analysis.
Main Results:
- Optimized UAE achieved a maximum FP yield of 2.182%.
- Significant structural differences were observed between FP and SFP, with SFP showing higher extraction yield.
- Both FP and SFP reduced hypertension and vascular injury, modulated gut microbiota, increased SCFAs, and enhanced intestinal barrier function, with SFP demonstrating more pronounced effects.
- Both polysaccharides inhibited the aortic TLR4/MyD88 pathway.
Conclusions:
- Stir-frying significantly modifies FT polysaccharide structure, enhancing their antihypertensive and gut-protective properties.
- SFP demonstrates superior efficacy in improving gut microbiome regulation, barrier function, and vascular outcomes compared to FP.
- The study highlights the value of traditional processing in enhancing the therapeutic potential of herbal medicine constituents.
