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Updated: Jun 24, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
Published on: January 14, 2016
Dynamic Assemblies in Genome Maintenance
Paras Gaur1,2, Maria Spies3,4
1Department of Biochemistry and Molecular Biology, University of Iowa Carver College of Medicine, Iowa City, IA, USA. paras-gaur@uiowa.edu.
Abstract:
The integrity of the human genome is continuously challenged by diverse endogenous and exogenous threats that damage DNA and disrupt its replication. When the replication machinery encounters such obstacles, including lesions or non-canonical DNA structures, it may stall, initiate repair, or activate specialized pathways to bypass the impediment. Maintaining replication progression requires a coordinated and dynamic assembly of numerous nucleoprotein complexes that recognize, process, and resolve DNA damage and replication stalling structures. This chapter highlights how proliferating cell nuclear antigen (PCNA), poly(ADP-ribose) polymerase 1 (PARP1), and non-canonical DNA structures are integrated into higher-order supramolecular complexes that stabilize, remodel, or resolve stalled or damaged replication forks. The molecular events carried out by these supramolecular complexes are essential for preserving genomic integrity in human cells. Moreover, many of the factors involved emerge as attractive therapeutic targets for diseases driven by genome instability, including cancer.
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