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CD44 expression on murine tissues

S J Kennel1, T K Lankford, L J Foote

  • 1Oak Ridge National Laboratory, TN 37831-8077.

Journal of Cell Science
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

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This study characterizes CD44 glycoprotein expression in mouse tissues, revealing distinct small and large forms in various organs and epithelia. The research highlights CD44

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • CD44 is a crucial cell surface glycoprotein involved in cell-cell interactions, particularly between lymphocytes, macrophages, and endothelium.
  • The CD44 protein exists in multiple isoforms generated through alternative mRNA splicing and post-translational modifications, suggesting diverse functional roles.
  • The specific functions of CD44 on epithelial cells and the distribution of its various forms in normal tissues remain incompletely understood.

Purpose of the Study:

  • To investigate the expression patterns and molecular forms of CD44 in various normal mouse tissues.
  • To utilize a newly isolated monoclonal antibody (mAb 133-13A) recognizing the P100 form of CD44 for tissue analysis.
  • To compare CD44 expression in normal tissues with that observed in murine lung tumor cells and human cell lines.

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Main Methods:

  • Isolation and characterization of monoclonal antibody (mAb) 133-13A against a 90-100 x 10(3) M(r) glycoprotein (P100) from murine lung tumor cells.
  • Amino acid sequence analysis to confirm that P100 is a form of CD44.
  • Quantitative analysis of CD44(P100) distribution across multiple normal mouse tissues using mAb 133-13A.

Main Results:

  • CD44(P100) expression is high in spleen, thymus, liver, intestine, uterus, and choroid plexus, as well as in the epithelia of adrenals, esophagus, and trachea.
  • In lymphoid tissues, the low molecular mass form (80-90 x 10(3) M(r)) predominates, consistent with findings in human lymphoid tissue.
  • Both small (~81 x 10(3) M(r)) and large forms of CD44 are found in basal epithelia of the esophagus and trachea and in salivary glands; however, only the small form is detected in the adrenal cortex epithelium and in studied murine lung and mammary carcinomas.

Conclusions:

  • The study demonstrates differential expression of CD44 forms across various mouse tissues and cell types.
  • Large forms of CD44 appear to be minor components in normal tissues, primarily localized to basal epithelia.
  • The low molecular mass CD44 found in epithelial tissues is likely associated with infiltrating lymphoid cells rather than epithelial cells themselves.