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Isolation of lung alveolar walls
Advances in Experimental Medicine and Biology
|January 1, 1977
Summary
Researchers developed a method to isolate alveolar wall tissue from peripheral lung tissue. This technique uses disintegration and filtration, providing a scalable source for lung tissue research.
Area of Science:
- Pulmonary Science
- Tissue Engineering
- Cell Biology
Background:
- Alveolar wall tissue is crucial for gas exchange in the lungs.
- Obtaining pure alveolar wall tissue for research has been challenging.
Purpose of the Study:
- To describe a novel method for isolating alveolar wall tissue.
- To demonstrate the feasibility of obtaining large quantities of this tissue.
Main Methods:
- Disintegration of peripheral lung tissue.
- Filtration of disintegrated tissue through an 80-mesh stainless steel screen.
Main Results:
- The filtration process yielded a filtrate containing tissue particles.
- These particles were identified as fragments of alveolar walls.
- The procedure efficiently produced substantial amounts of alveolar wall tissue.
Conclusions:
- This method provides a reliable and scalable source of alveolar wall tissue.
- The isolated tissue is suitable for further research applications.
- This technique simplifies the procurement of key lung components for scientific study.