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Immunochemical methods for fumonisins

F S Chu1

  • 1Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706, USA.

Advances in Experimental Medicine and Biology
|January 1, 1996
PubMed
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Researchers developed antibodies for detecting fumonisins (Fm), a mycotoxin. Immunoassays show promise for screening corn and feed, but matrix interferences require cleanup methods for accurate fumonisin detection.

Area of Science:

  • Mycotoxicology
  • Immunochemistry
  • Analytical Chemistry

Background:

  • Fumonisins (Fm) are mycotoxins that contaminate food and feed, posing health risks.
  • Immunoassays utilizing antibodies are crucial for detecting fumonisins.
  • Challenges exist in accurately quantifying fumonisins due to sample matrix interferences.

Purpose of the Study:

  • To review antibody generation strategies for fumonisin detection.
  • To discuss the specificity and sensitivity of various fumonisin immunoassays.
  • To explore methods for overcoming interferences and enhancing immunoassay performance.

Main Methods:

  • Development of monoclonal and polyclonal antibodies against fumonisins and their hydrolyzed forms.
  • Application of antibodies in various immunoassay formats for fumonisin quantification.

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  • Utilization of antibodies for generating anti-idiotype antibodies and affinity columns for sample cleanup.

    • Main Results:

    • Antibodies can detect fumonisins (FmB1) in standard solutions within a broad range (5-5000 ng/mL).
    • Immunoassays without cleanup show significant matrix interferences, limiting screening to 0.5-10 ppm in corn/feed.
    • Antibodies are versatile, enabling use in immunohistology and affinity chromatography for sample purification.

    Conclusions:

    • Antibody-based immunoassays are valuable tools for fumonisin detection and screening.
    • Effective sample cleanup strategies are essential to mitigate matrix effects and improve assay accuracy.
    • Ongoing research focuses on enhancing antibody specificity and immunoassay sensitivity for reliable fumonisin analysis.